Validating internal controls for quantitative plant gene expression studies

28-Aug-2020 12:23

However, accurate measurement of gene expression with this method relies on the choice of a valid reference for data normalization.

Studies rarely verify that gene expression levels for reference genes are adequately consistent among the...

Transcription factor genes represent a sizable fraction of the genomes of all eukaryotic organisms, including higher plants [7].

Various high throughput techniques that allow the accurate quantification of expression levels (transcript abundance) of hundreds or thousands of genes are currently available [1].